Collaborative Research and Shared Equipments

Experimental Equipments
Room114
 
High-speed refrigerated centrifuge (COE)
  Use for biomaterial separation, fractionation and so on. Various size of rotors are set sup.  
High-speed refrigerated centrifuge (COE)

 
Freeze dry system (COE)
  Freeze, dry and Concentrate biomaterials.  
Freeze dry system (COE)

 
High-performance liquid chromatography (AKTA FPLC)
  This is the system of high-performance liquid chromatography optimized purification of proteins make use of gel filtration, ion exchange and affinity mode. This system is useful for purification of recombinant protein.  
High-performance liquid chromatography (AKTA FPLC)

 
High-performance liquid chromatography
(Two-dimensional HPLC)
  This is the system of high-performance liquid chromatography for proteome analysis. This system have an ability to do two step purification of protein by chromatofocusing and reverse-phase chromatography  
High-performance liquid chromatography(Two-dimensional HPLC)
Room115
 
Benchtop ultracentrifuge
  Use for biomaterial separation, fractionation and so on.  
Benchtop ultracentrifuge

 
UV/Visible spectrophotometers
  Use for quantification of protein and nucleic acids.  
UV/Visible spectrophotometers
Molecular Medicine
 
ARVO X3 Multilabel Plate Reader 2030 (PerkinElmer)
with dispenser module including two syringes
  ARVO X3 Plate Reader includes luminescence, fluorescence intensity and UV-absorbance technologies. protein assay.  
ARVO X3 Multilabel Plate Reader 2030 (PerkinElmer) with dispenser module including two syringes

 
Bio-Plex 200 System (Bio-RAD) with Bio-Plex Manager
software version 6.0
  Bio-Plex assays are bead-based assays that can be performed in a mixed array.  
Bio-Plex 200 System (Bio-RAD) with Bio-Plex Manager software version 6.0
Genome Repair
 
HiSeq2500 system (Illumina®)
  Highest throughput next generation sequencer in practical use. 600 Gb/week data can be obtained at standard run mode, and 60 Gb/2days data can be at rapid run mode. Essential for genome research.  
HiSeq2500 system (Illumina®)

 
MiSeq system (Illumina®)
  Personal type next generation sequencer. Throughput itself is smaller than HiSeq system, but can read long fragment due to rapid reaction. This system is suitable for genome research required small data volume such mutation screening for target gene.  
MiSeq system (Illumina®)
Human Genetics
 
HiSeq2500 system (Illumina®)
  Highest throughput next generation sequencer in practical use. 600 Gb/week data can be obtained at standard run mode, and 60 Gb/2days data can be at rapid run mode. Essential for genome research.  
HiSeq2500 system (Illumina®)

 

 
Affymetrix GeneChip System (Affymetrix®)
  This system makes it possible to genotype over one million single nucl eotide polymorphisms (SNP) in one individual, and to detect the region with copy number changes/variation in the genome.  
Affymetrix GeneChip System (Affymetrix®)

 
3730 genetic analyzer (AppliedBiosystems)
  Capillary type autosequencer. Fourty-eight samples can be analyzed in one sequence run.  
3730 genetic analyzer (AppliedBiosystems)

 
Covaris Acourstic Solubilizer Model S2 (Covaris®)
  The versatility of the S20 Focused-ultrasonicator makes it possible to bring the advantages of Adaptive Focused Acoustic technologies to numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization.  
Covaris Acourstic Solubilizer Model S2 (Covaris®)

 
3130xl genetic analyzer (AppliedBiosystems)
  Capillary type autosequencer. Sixteen samples can be analyzed in one sequence run/hour. Fragment analyze can be applied for Short Tandem Repeat Polymorphism (STRP).  
3130xl genetic analyzer (AppliedBiosystems)

 
IonProtonTM system (LifeTechnologies)
  Semiconductor sequencer. Base call is performed by detection of pH change due to proton release during DNA synthesis. A thouthand dollar genome sequence for human genome can be achieved by high density ion detecting chip and accurate base calling.  
IonProton\sl201\slmult0 TM system (LifeTechnologies)

 
5500 SOLiD system(LefeTechnologies)
  5500 SOLiD system make it possible 80 Giga base pair sequence throughput / FlowChip / run. 5500 SOLiD system applied unique sequencing method by ligation reaction on FlowChip after emulsion PCR.  
5500 SOLiD system(LefeTechnologies)

 
LightCycler® 480 Real-Time PCR system
(Roche Applied Science)
  The LightCycler® 480 Real-Time PCR System is a 384 multiwellplate based real-time PCR platform which is used for highly accurate qualitative and quantitative detection of nucleic acids, genotyping and mutation screening.  
LightCycler® 480 Real-Time PCR system (Roche Applied Science)

 
Caliper LabChip GX system (Caliper, Perkin Elmer)
  Auto electrophoresis system for DNA/RNA. 96 samples are run in 1.5 hours. GX system makes it possible to detect 5 bp differences in 1 kb length fragment and to quantify the amount of nucleic acids within variable size rage.  
Caliper LabChip GX system (Caliper, Perkin Elmer)

 
Multi-Beads Shocker (YASUI KIKAI)
  Hard samples in room temperature or liquid-nitrogen frozen samples can be crumbed up by hammering and shearing power generated by three-dimensional 8 figure movement. It is very useful for DNA/RNA extraction from samples.  
Multi-Beads Shocker (YASUI KIKAI)

 
ImageQuant LAS 4000mini Lumino Image Analyzer
(GE healthcare)
  CCD image system to detect chemical light emission and fluorescent emission. This system is the highest sensitive system and very useful for Western blot analysis.  
ImageQuant LAS 4000mini Lumino Image Analyzer(GE healthcare)
Radiation Medical Science
 
BD FACSJazz Cell Sorter (BD)
  The BD FACSJAzz cell sorter is a stream-inair sorter and capable of two-way sorting with 5-mL and 15-mL tubes. Direct sorting into multi-well plates (6, 24, 96, and 384-well plates) and slides is also possible.
488 nm laser; the number of fluorescence detectors/filters: 4; nozzle: 100 microM; an average threshol rate: 10,000 events/sec.
 
BD FACSJazz Cell Sorter (BD)
Tumor and Diagnostic Pathology
 
HS All-in-One Fluorescence
Microscope BZ-9000 Series
  Built-in sample enclosure eliminates the need for a darkroom. The image capturing feature can be fully controlled with the electronic XYZ axes stage, electronic optical zoom, electronic shutter and electronic filter turret.  
  HS All-in-One Fluorescence Microscope BZ-9000 Series

 
Alpha Spectroscopy System with Frisch Grid
Chamber Detector
  The most sensitive of Alpha detectors(100% relative efficiency in 2πsteradians)
- Automated and electropneumatically controlled measurement cycle
- Resolution: < 35 keV with Pu-239
- Background: < 1 count/h between 4 and 6 MeV
 
Alpha Spectroscopy System with Frisch Grid Chamber Detector

 
Autostainer for immunohistochemistry
(Ventana Discovery XT)
  The DISCOVERY XT sysem bringsautomation, reproducibility and productivity to immunohistochemistry (IHC) slide preparation and processing. Automate any one, or all preparation steps including deparaffinization, antigen retrieval, blocking and staining.  
Autostainer for immunohistochemistry (Ventana Discovery XT)

 
QIAcube:Nucleic acid extraction and purification
equipment (QIAGEN)
  Fully automated purification of nucleic acids using QIAGEN spin-column kits.
Extract and purify DNA or RNA from up to 12 samples per run. The standardized, automated purification procedure helps to eliminate human error, providing results that are comparable between experiments and labs.
 
QIAcube:Nucleic acid extraction and purification equipment (QIAGEN)
Hematology
 
The MVE 1500 Series -190℃ freezers
  This system provides cryogenic storage for up to 42,000 1.2 / 2.0 ml vials. These freezers provide maximum storage density and provide the industry's longest hold time.  
The MVE 1500 Series -190℃ freezers
Human Counter Room
 
Whole-body counter
  Whole-body counter (WBC) is a special device to measure internal body burden of radionuclides. By the ultrasensitive radiation detectors (NaI (Tl) scintillators), WBC can detect radiation doses released from human bodies. Since WBC can detect even minimal doses, it is necessary to exclude the effects of natural background radiation by shielding (20cm thickness iron). K-40 and Cs-137 are mainly detected radionuclides by WBC.WBC was launched at Nagasaki University in 1968, and had been used for the measurement of internal body burden of Cs-137 among populations residing in Nagasaki city for 15 years. Also, since the accident at Chernobyl Nuclear Power Plant in Ukraine, it has been used for the measurement of internal body burden of radionuclides among residents around Chernobyl. Now Nagasaki University hospital is nominated as secondary medical facility in case of radiation emergencies. Our WBC is available for the evaluation of internal body burden of radionuclides during such emergencies.  
Whole-body counter
Radiation Exposure Room
 
X-ray system
  Our X-ray system is a device to radiate X-ray on the cultured cells or the laboratory animals such as rats and mice to examine the effect of radiation to living organisms.
It is composed of an X-ray tube, an X-ray generator, a high voltage generator, and a cooling unit, etc. The X-ray system is placed in the restricted area.
 
X-ray system
Other Facilities
Atomic Bomb Disease Exhibition Rooms
 

Atomic Bomb Disease Institute
 
Computer Room (3rd floor) Training Room (4th floor) Community Center (3rd Floor)
Computer Room (3rd floor) Training Room (4th floor) Community Center (3rd Floor)